Chikungunya virus (CHIKV) presents a major burden on healthcare systems worldwide, but specific treatment remains unavailable.Attachment and fusion of CHIKV to the host cell membrane is mediated by the E1/E2 protein spikes.We used an in vitro single-particle fusion iphone 13 pro max price winnipeg assay to study the effect of the potent, neutralizing antibody CHK-152 on CHIKV binding and fusion.We find that CHK-152 shields the virions, inhibiting interaction with the target membrane and inhibiting fusion.The analysis of the ratio of bound antibodies to epitopes implied that CHIKV fusion is a highly cooperative process.
Further, dissociation of the antibody at lower pH results in a finely balanced kinetic competition between inhibition and fusion, suggesting a window of opportunity for the spike proteins to act and mediate fusion, bekindtopets.com even in the presence of the antibody.